Slot: pcr conditions (pcr_cond)

Description of reaction conditions and components of polymerase chain reaction performed during library preparation.

URI: MIXS:0000049

Applicable Classes

Name	Description	Modifies Slot
LibraryPreparation		yes

Properties

• Range: String

Examples

Value
initial denaturation:94_3;annealing:50_1;elongation:72_1.5;final elongation:72_10;35
initial denaturation:94degC_1.5min

Identifier and Mapping Information

Annotations

property	value
Expected_value	initial denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final elongation:degrees_minutes;total cycles

Schema Source

• from schema: https://w3id.org/nmdc/nmdc

Mappings

Mapping Type	Mapped Value
self	MIXS:0000049

LinkML Source

name: pcr_cond
annotations:
  Expected_value:
    tag: Expected_value
    value: initial denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final
      elongation:degrees_minutes;total cycles
description: Description of reaction conditions and components of polymerase chain
  reaction performed during library preparation.
title: pcr conditions
examples:
- value: initial denaturation:94_3;annealing:50_1;elongation:72_1.5;final elongation:72_10;35
- value: initial denaturation:94degC_1.5min
from_schema: https://w3id.org/nmdc/nmdc
rank: 1000
keywords:
- condition
- pcr
string_serialization: initial denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final
  elongation:degrees_minutes;total cycles
slot_uri: MIXS:0000049
alias: pcr_cond
domain_of:
- LibraryPreparation
range: string