Slot: pcr primers (pcr_primers)

PCR primers that were used to amplify the sequence of the targeted gene, locus or subfragment. This field should contain all the primers used for a single PCR reaction if multiple forward or reverse primers are present in a single PCR reaction. The primer sequence should be reported in uppercase letters

URI: MIXS:0000046

Inheritance

• sequencing_field
  • pcr_primers

Applicable Classes

Name	Description	Modifies Slot
LibraryPreparation		no

Properties

• Range: TextValue

Aliases

• pcr primers

Examples

Value
FWD:GTGCCAGCMGCCGCGGTAA;REV:GGACTACHVGGGTWTCTAAT

Identifier and Mapping Information

Annotations

property	value
expected_value	FWD: forward primer sequence;REV:reverse primer sequence

Schema Source

• from schema: https://w3id.org/nmdc/nmdc

Mappings

Mapping Type	Mapped Value
self	MIXS:0000046
native	nmdc:pcr_primers

LinkML Source

name: pcr_primers
annotations:
  expected_value:
    tag: expected_value
    value: 'FWD: forward primer sequence;REV:reverse primer sequence'
description: PCR primers that were used to amplify the sequence of the targeted gene,
  locus or subfragment. This field should contain all the primers used for a single
  PCR reaction if multiple forward or reverse primers are present in a single PCR
  reaction. The primer sequence should be reported in uppercase letters
title: pcr primers
examples:
- value: FWD:GTGCCAGCMGCCGCGGTAA;REV:GGACTACHVGGGTWTCTAAT
from_schema: https://w3id.org/nmdc/nmdc
aliases:
- pcr primers
rank: 1000
is_a: sequencing field
string_serialization: FWD:{dna};REV:{dna}
slot_uri: MIXS:0000046
alias: pcr_primers
domain_of:
- LibraryPreparation
range: TextValue
multivalued: false