If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided. (Example: 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles)
URI: MIXS:0000099
Inheritance
Applicable Classes
Properties
Aliases
- organism count qPCR information
Examples
Annotations
property |
value |
expected_value |
gene name;FWD:forward primer sequence;REV:reverse primer sequence;initial denaturation:degrees_minutes;denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final elongation:degrees_minutes; total cycles |
Schema Source
- from schema: https://example.com/nmdc_submission_schema
LinkML Source
name: org_count_qpcr_info
annotations:
expected_value:
tag: expected_value
value: gene name;FWD:forward primer sequence;REV:reverse primer sequence;initial
denaturation:degrees_minutes;denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final
elongation:degrees_minutes; total cycles
preferred_unit:
tag: preferred_unit
value: number of cells per gram (or ml or cm^2)
occurrence:
tag: occurrence
value: '1'
description: 'If qpcr was used for the cell count, the target gene name, the primer
sequence and the cycling conditions should also be provided. (Example: 16S rrna;
FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min;
annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min;
30 cycles)'
title: organism count qPCR information
examples:
- value: ''
from_schema: https://example.com/nmdc_submission_schema
aliases:
- organism count qPCR information
rank: 1000
is_a: core field
string_serialization: '{text};FWD:{dna};REV:{dna};initial denaturation:degrees_minutes;denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final
elongation:degrees_minutes; total cycles'
slot_uri: MIXS:0000099
multivalued: false
alias: org_count_qpcr_info
domain_of:
- HcrCoresInterface
- HcrFluidsSwabsInterface
range: string