Enum: LibraryStrategyEnum

Sequencing strategy used for library preparation

URI: LibraryStrategyEnum

Permissible Values

Value	Meaning	Description
WGA	None	Whole genome amplification followed by random sequencing
WGS	None	Random sequencing of the whole genome
RNA-Seq	None	Random sequencing of whole transcriptome
AMPLICON	None	Sequencing of overlapping or distinct PCR or RT-PCR products

Slots

Name	Description
library_strategy	Sequencing technique intended for this library

Comments

• NMDC intentionally supports a curated subset of the INSDC library strategy vocabulary. The complete controlled vocabulary is defined in the referenced SRA.experiment.xsd; values can be added as needed.

See Also

• https://github.com/enasequence/webin-xml/blob/2.1.0/src/main/resources/uk/ac/ebi/ena/sra/schema/SRA.experiment.xsd

Identifier and Mapping Information

Schema Source

• from schema: https://w3id.org/nmdc/nmdc

LinkML Source

name: LibraryStrategyEnum
description: Sequencing strategy used for library preparation
comments:
- NMDC intentionally supports a curated subset of the INSDC library strategy vocabulary.
  The complete controlled vocabulary is defined in the referenced SRA.experiment.xsd;
  values can be added as needed.
from_schema: https://w3id.org/nmdc/nmdc
see_also:
- https://github.com/enasequence/webin-xml/blob/2.1.0/src/main/resources/uk/ac/ebi/ena/sra/schema/SRA.experiment.xsd
rank: 1000
permissible_values:
  WGA:
    text: WGA
    description: Whole genome amplification followed by random sequencing.
    title: Whole Genome Amplification
    see_also:
    - pubmed:1631067
    - pubmed:8962113
  WGS:
    text: WGS
    description: Random sequencing of the whole genome.
    title: Whole Genome Sequencing
    see_also:
    - pubmed:10731132
  RNA-Seq:
    text: RNA-Seq
    description: Random sequencing of whole transcriptome
    see_also:
    - pubmed:18611170
    aliases:
    - Whole Transcriptome Shotgun Sequencing
    - WTSS
  AMPLICON:
    text: AMPLICON
    description: Sequencing of overlapping or distinct PCR or RT-PCR products
    comments:
    - metagenomic community profiling using SSU rRNA, for example