Class: JGI Isolate Transcriptome (JgiIsolateTranscriptomeInterface)
JGI submission metadata for isolate TRANSCRIPTOME sequencing projects. Shared JGI logistics plus the RNA experiment collection date.
URI: nmdc_sub_schema:JgiIsolateTranscriptomeInterface
classDiagram
class JgiIsolateTranscriptomeInterface
click JgiIsolateTranscriptomeInterface href "../JgiIsolateTranscriptomeInterface/"
DhMultiviewCommonColumnsMixin <|-- JgiIsolateTranscriptomeInterface
click DhMultiviewCommonColumnsMixin href "../DhMultiviewCommonColumnsMixin/"
JgiIsolateCommonMixin <|-- JgiIsolateTranscriptomeInterface
click JgiIsolateCommonMixin href "../JgiIsolateCommonMixin/"
DhInterface <|-- JgiIsolateTranscriptomeInterface
click DhInterface href "../DhInterface/"
JgiIsolateTranscriptomeInterface : analysis_type
JgiIsolateTranscriptomeInterface --> "1..*" AnalysisTypeEnum : analysis_type
click AnalysisTypeEnum href "../AnalysisTypeEnum/"
JgiIsolateTranscriptomeInterface : biosafety_mat_cat
JgiIsolateTranscriptomeInterface --> "1" JgiIsolateMaterialKindEnum : biosafety_mat_cat
click JgiIsolateMaterialKindEnum href "../JgiIsolateMaterialKindEnum/"
JgiIsolateTranscriptomeInterface : collection_site_or_growth_conditions
JgiIsolateTranscriptomeInterface : cont_type
JgiIsolateTranscriptomeInterface --> "1" JgiContTypeEnum : cont_type
click JgiContTypeEnum href "../JgiContTypeEnum/"
JgiIsolateTranscriptomeInterface : cont_well
JgiIsolateTranscriptomeInterface : container_name
JgiIsolateTranscriptomeInterface : dnase
JgiIsolateTranscriptomeInterface --> "1" YesNoEnum : dnase
click YesNoEnum href "../YesNoEnum/"
JgiIsolateTranscriptomeInterface : host_genus
JgiIsolateTranscriptomeInterface : host_species
JgiIsolateTranscriptomeInterface : host_strain
JgiIsolateTranscriptomeInterface : host_taxid
JgiIsolateTranscriptomeInterface : isolate_meth
JgiIsolateTranscriptomeInterface : jgi_project_pi
JgiIsolateTranscriptomeInterface : jgi_proposal_id
JgiIsolateTranscriptomeInterface : jgi_samp_id
JgiIsolateTranscriptomeInterface : jgi_sample_contact
JgiIsolateTranscriptomeInterface : jgi_sample_format
JgiIsolateTranscriptomeInterface --> "1" JGISampleFormatEnum : jgi_sample_format
click JGISampleFormatEnum href "../JGISampleFormatEnum/"
JgiIsolateTranscriptomeInterface : jgi_sample_name
JgiIsolateTranscriptomeInterface : jgi_sample_volume
JgiIsolateTranscriptomeInterface : jgi_seq_project
JgiIsolateTranscriptomeInterface : jgi_seq_project_name
JgiIsolateTranscriptomeInterface : nuc_acid_absorb1
JgiIsolateTranscriptomeInterface : nuc_acid_absorb2
JgiIsolateTranscriptomeInterface : nuc_acid_concentration
JgiIsolateTranscriptomeInterface : reference_genome
JgiIsolateTranscriptomeInterface : replicate_group
JgiIsolateTranscriptomeInterface : rna_collection_date
JgiIsolateTranscriptomeInterface : samp_name
JgiIsolateTranscriptomeInterface : sample_isolated_from
JgiIsolateTranscriptomeInterface : source_mat_id
Inheritance
- DhInterface
- JgiIsolateTranscriptomeInterface [ DhMultiviewCommonColumnsMixin JgiIsolateCommonMixin]
Slots
| Name | Cardinality and Range | Description | Inheritance |
|---|---|---|---|
| rna_collection_date | 1 String |
Date the RNA sample was collected or the experiment was performed, in YYYY-MM... | direct |
| analysis_type | 1..* AnalysisTypeEnum |
Select all the data types associated or available for this biosample | DhMultiviewCommonColumnsMixin |
| samp_name | 1 String |
A local identifier or name that for the material sample collected | DhMultiviewCommonColumnsMixin |
| source_mat_id | 0..1 String |
A globally unique identifier assigned to the parent sample or sample that is ... | DhMultiviewCommonColumnsMixin |
| replicate_group | 0..1 String |
Samples that are biological replicates should have the same group name | JgiIsolateCommonMixin |
| jgi_samp_id | 1 String |
JgiIsolateCommonMixin | |
| jgi_sample_name | 1 String |
Give the JGI sample a name that is meaningful to you | JgiIsolateCommonMixin |
| jgi_seq_project | 1 Float |
JgiIsolateCommonMixin | |
| jgi_seq_project_name | 1 String |
JgiIsolateCommonMixin | |
| jgi_sample_contact | 1 String |
JgiIsolateCommonMixin | |
| jgi_project_pi | 1 String |
JgiIsolateCommonMixin | |
| jgi_proposal_id | 1 String |
JgiIsolateCommonMixin | |
| nuc_acid_absorb1 | 0..1 recommended Float |
260/280 measurement of nucleic acid sample purity | JgiIsolateCommonMixin |
| nuc_acid_absorb2 | 0..1 recommended Float |
260/230 measurement of nucleic acid sample purity | JgiIsolateCommonMixin |
| nuc_acid_concentration | 1 Float |
JgiIsolateCommonMixin | |
| jgi_sample_volume | 1 Float |
JgiIsolateCommonMixin | |
| cont_type | 1 JgiContTypeEnum |
Tube or plate (96-well) | JgiIsolateCommonMixin |
| cont_well | 0..1 recommended String |
JgiIsolateCommonMixin | |
| container_name | 1 String |
JgiIsolateCommonMixin | |
| jgi_sample_format | 1 JGISampleFormatEnum |
Solution in which the JGI sample has been suspended | JgiIsolateCommonMixin |
| dnase | 1 YesNoEnum |
JgiIsolateCommonMixin | |
| biosafety_mat_cat | 1 JgiIsolateMaterialKindEnum |
JGI material category, labeled "Biosafety Material Category" on the form and ... | JgiIsolateCommonMixin |
| isolate_meth | 1 String |
Method, protocol, or kit used to extract DNA or RNA from the sample | JgiIsolateCommonMixin |
| reference_genome | 0..1 String |
Genome record to use as a reference for read alignment or assembly scaffoldin... | JgiIsolateCommonMixin |
| sample_isolated_from | 1 String |
Free-text description of what the organism was isolated from | JgiIsolateCommonMixin |
| collection_site_or_growth_conditions | 0..1 String |
Free-text description of the collection site or growth conditions for the iso... | JgiIsolateCommonMixin |
| host_taxid | 0..1 String |
NCBI taxon id of the host, e | JgiIsolateCommonMixin |
| host_genus | 0..1 String |
Genus of the host organism that the sample was collected from | JgiIsolateCommonMixin |
| host_species | 0..1 String |
Species of the host organism that the sample was collected from | JgiIsolateCommonMixin |
| host_strain | 0..1 String |
Strain of the host organism that the sample was collected from | JgiIsolateCommonMixin |
Usages
| used by | used in | type | used |
|---|---|---|---|
| SampleData | jgi_isolate_transcriptome_data | range | JgiIsolateTranscriptomeInterface |
Identifier and Mapping Information
Annotations
| property | value |
|---|---|
| excel_worksheet_name | JGI Isolate Transcriptome |
Schema Source
- from schema: https://example.com/nmdc_submission_schema
Mappings
| Mapping Type | Mapped Value |
|---|---|
| self | nmdc_sub_schema:JgiIsolateTranscriptomeInterface |
| native | nmdc_sub_schema:JgiIsolateTranscriptomeInterface |
LinkML Source
Direct
name: JgiIsolateTranscriptomeInterface
annotations:
excel_worksheet_name:
tag: excel_worksheet_name
value: JGI Isolate Transcriptome
description: JGI submission metadata for isolate TRANSCRIPTOME sequencing projects.
Shared JGI logistics plus the RNA experiment collection date.
title: JGI Isolate Transcriptome
from_schema: https://example.com/nmdc_submission_schema
is_a: DhInterface
mixins:
- DhMultiviewCommonColumnsMixin
- JgiIsolateCommonMixin
slots:
- rna_collection_date
slot_usage:
rna_collection_date:
name: rna_collection_date
slot_group: jgi_isolate_section
required: true
Induced
name: JgiIsolateTranscriptomeInterface
annotations:
excel_worksheet_name:
tag: excel_worksheet_name
value: JGI Isolate Transcriptome
description: JGI submission metadata for isolate TRANSCRIPTOME sequencing projects.
Shared JGI logistics plus the RNA experiment collection date.
title: JGI Isolate Transcriptome
from_schema: https://example.com/nmdc_submission_schema
is_a: DhInterface
mixins:
- DhMultiviewCommonColumnsMixin
- JgiIsolateCommonMixin
slot_usage:
rna_collection_date:
name: rna_collection_date
slot_group: jgi_isolate_section
required: true
attributes:
rna_collection_date:
name: rna_collection_date
description: Date the RNA sample was collected or the experiment was performed,
in YYYY-MM-DD format. For RNA projects, use the experiment date rather than
the original field collection date.
title: Collection date
examples:
- value: '2017-01-20'
- value: '2021-06-15'
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Collection Year:
literal_form: Collection Year
predicate: RELATED_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
Collection Month:
literal_form: Collection Month
predicate: RELATED_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
Collection Day:
literal_form: Collection Day
predicate: RELATED_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 43
alias: rna_collection_date
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiIsolateTranscriptomeInterface
slot_group: jgi_isolate_section
range: string
required: true
pattern: ^[12]\d{3}-(?:0[1-9]|1[0-2])-(?:0[1-9]|[12]\d|3[01])$
analysis_type:
name: analysis_type
description: Select all the data types associated or available for this biosample
title: analysis/data type
comments:
- MIxS:investigation_type was included as a `see_also` but that term doesn't resolve
any more
examples:
- value: metagenomics; metabolomics; metaproteomics
from_schema: https://example.com/nmdc_submission_schema
rank: 3
alias: analysis_type
owner: JgiIsolateTranscriptomeInterface
domain_of:
- DhMultiviewCommonColumnsMixin
slot_group: sample_id_section
range: AnalysisTypeEnum
required: true
multivalued: true
samp_name:
name: samp_name
annotations:
Preferred_unit:
tag: Preferred_unit
value: ''
description: A local identifier or name that for the material sample collected.
Refers to the original material collected or to any derived sub-samples.
title: sample name
comments:
- It can have any format, but we suggest that you make it concise, unique and
consistent within your lab, and as informative as possible.
- If multiple sample environments (soil, water, etc) are used, these values must
be unique across all environments.
examples:
- value: Rock core CB1178(5-6) from NSW
from_schema: https://example.com/nmdc_submission_schema
rank: 1
keywords:
- sample
slot_uri: MIXS:0001107
identifier: true
alias: samp_name
owner: JgiIsolateTranscriptomeInterface
domain_of:
- DhMultiviewCommonColumnsMixin
slot_group: sample_id_section
range: string
required: true
source_mat_id:
name: source_mat_id
annotations:
Expected_value:
tag: Expected_value
value: 'for cultures of microorganisms: identifiers for two culture collections;
for other material a unique arbitrary identifer'
description: A globally unique identifier assigned to the parent sample or sample
that is the source of this sample.
title: source material identifier
todos:
- Currently, the comments say to use UUIDs. However, if we implement assigning
NMDC identifiers with the minter we dont need to require a GUID. It can be an
optional field to fill out only if they already have a resolvable ID.
comments:
- Identifiers must be prefixed. Possible FAIR prefixes are IGSNs (http://www.geosamples.org/getigsn),
NCBI biosample accession numbers, ARK identifiers (https://arks.org/). These
IDs enable linking to derived analytes and subsamples. If you have not assigned
FAIR identifiers to your samples, you can generate UUIDs (https://www.uuidgenerator.net/).
- 'Prefixed FAIR identifiers are preferred where available: `igsn` for International
Generic Sample Numbers (http://www.geosamples.org/getigsn), `biosample` for
NCBI biosample accession IDs, `gold` for GOLD identifiers. The format is not
constrained, so culture collection identifiers such as "DSM 6724" or "ATCC 5680"
are also accepted.'
examples:
- value: igsn:AU1243
- value: biosample:SAMEA2397676
from_schema: https://example.com/nmdc_submission_schema
rank: 2
keywords:
- identifier
- material
- source
slot_uri: MIXS:0000026
alias: source_mat_id
owner: JgiIsolateTranscriptomeInterface
domain_of:
- DhMultiviewCommonColumnsMixin
- IsolateInterface
slot_group: sample_id_section
range: string
multivalued: false
replicate_group:
name: replicate_group
description: Samples that are biological replicates should have the same group
name. If your project does not contain biological replicates, give each sample
its own group name.
title: Biological replicate/sample group Name
examples:
- value: SampleGroup1
from_schema: https://example.com/nmdc_submission_schema
rank: 5
alias: replicate_group
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
jgi_samp_id:
name: jgi_samp_id
title: JGI sample ID
comments:
- Do not edit these values. A template will be provided by NMDC in which these
values have been pre-filled. If you do not have these values, please contact
your NMDC POC & your JGI PM.
examples:
- value: '187654'
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_samp_id
- rna_samp_id
rank: 3
alias: jgi_samp_id
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
jgi_sample_name:
name: jgi_sample_name
description: Give the JGI sample a name that is meaningful to you. Sample names
must be unique across all JGI projects and contain a-z, A-Z, 0-9, - and _ only.
title: JGI sample name
examples:
- value: JGI_pond_041618
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_sample_name
- rna_sample_name
rank: 4
alias: jgi_sample_name
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
pattern: ^[-_.a-zA-Z0-9]*$
jgi_seq_project:
name: jgi_seq_project
title: JGI seq project ID
comments:
- Do not edit these values. A template will be provided by NMDC in which these
values have been pre-filled. If you do not have these values, please contact
your NMDC POC & your JGI PM.
examples:
- value: '1191234'
from_schema: https://example.com/nmdc_submission_schema
aliases:
- Seq Project ID
- dna_seq_project
- rna_seq_project
rank: 1
alias: jgi_seq_project
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: float
required: true
jgi_seq_project_name:
name: jgi_seq_project_name
title: JGI seq project name
comments:
- Do not edit these values. A template will be provided by NMDC in which these
values have been pre-filled. If you do not have these values, please contact
your NMDC POC & your JGI PM.
examples:
- value: JGI Pond metagenomics
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_seq_project_name
- rna_seq_project_name
rank: 2
alias: jgi_seq_project_name
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
jgi_sample_contact:
name: jgi_sample_contact
title: DNA sample contact
examples:
- value: Jordan James
from_schema: https://example.com/nmdc_submission_schema
rank: 18
alias: jgi_sample_contact
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
jgi_project_pi:
name: jgi_project_pi
title: JGI sequencing project PI
examples:
- value: Jordan James
from_schema: https://example.com/nmdc_submission_schema
rank: 19
alias: jgi_project_pi
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
jgi_proposal_id:
name: jgi_proposal_id
title: JGI proposal number
examples:
- value: PR123456
- value: '123456'
from_schema: https://example.com/nmdc_submission_schema
rank: 20
alias: jgi_proposal_id
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
pattern: ^[A-Z0-9]+$
nuc_acid_absorb1:
name: nuc_acid_absorb1
description: 260/280 measurement of nucleic acid sample purity
title: nucleic acid absorbance 260/280
comments:
- Recommended value is between 1 and 3.
examples:
- value: '2.02'
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_absorb1
- rna_absorb1
rank: 7
alias: nuc_acid_absorb1
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: float
recommended: true
minimum_value: 0.0001
nuc_acid_absorb2:
name: nuc_acid_absorb2
description: 260/230 measurement of nucleic acid sample purity
title: nucleic acid absorbance 260/230
comments:
- Recommended value is between 1 and 3.
examples:
- value: '2.02'
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_absorb2
- nuc_acid_absorb2
rank: 9
alias: nuc_acid_absorb2
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: float
recommended: true
minimum_value: 0.0001
nuc_acid_concentration:
name: nuc_acid_concentration
title: nucleic acid concentration in ng/ul
comments:
- Units must be in ng/uL. Enter the numerical part only. Must be calculated using
a fluorometric method. Acceptable values are greater than zero and at most 2000.
examples:
- value: '100'
from_schema: https://example.com/nmdc_submission_schema
see_also:
- nmdc:nucleic_acid_concentration
aliases:
- dna_concentration
- rna_concentration
rank: 6
alias: nuc_acid_concentration
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: float
required: true
minimum_value: 0.0001
maximum_value: 2000
jgi_sample_volume:
name: jgi_sample_volume
title: JGI sample volume in ul
comments:
- Units must be in uL. Enter the numerical part only. Value must be greater than
zero and at most 1000. This form accepts values < 25, but JGI may refuse to
process them unless permission has been granted by a project manager.
examples:
- value: '25'
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_volume
- rna_volume
rank: 8
alias: jgi_sample_volume
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: float
required: true
minimum_value: 0.0001
maximum_value: 1000
cont_type:
name: cont_type
description: Tube or plate (96-well)
title: JGI container type
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_cont_type
- rna_cont_type
rank: 11
alias: cont_type
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: JgiContTypeEnum
required: true
cont_well:
name: cont_well
title: plate position
comments:
- Required when 'plate' is selected for container type.
- Leave blank if the sample will be shipped in a tube.
- JGI will not process samples in corner wells, so A1, A12, H1 and H12 will not
pass validation.
- Fill plate in column-wise format. B1-G1,A2-H2,A3-D3 (NOT A2-A11,B1-B8).
examples:
- value: B2
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_cont_well
- rna_cont_well
rank: 12
alias: cont_well
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
recommended: true
pattern: ^(?!A1$|A12$|H1$|H12$)(([A-H][1-9])|([A-H]1[0-2]))$
container_name:
name: container_name
title: container name
comments:
- Must be unique across all tubes and plates, and <20 characters.
- All samples in a plate should have the same plate name.
examples:
- value: Pond_MT_041618
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_container_id
- rna_container_id
rank: 10
alias: container_name
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
pattern: ^[-_.a-zA-Z0-9]{1,20}$
jgi_sample_format:
name: jgi_sample_format
description: Solution in which the JGI sample has been suspended
title: JGI sample format
examples:
- value: Water
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_sample_format
- rna_sample_format
rank: 13
alias: jgi_sample_format
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: JGISampleFormatEnum
required: true
dnase:
name: dnase
title: DNase treatment
comments:
- Note DNase treatment is required for all RNA samples.
examples:
- value: 'no'
from_schema: https://example.com/nmdc_submission_schema
aliases:
- dna_dnase
- dnase_rna
rank: 15
alias: dnase
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: YesNoEnum
required: true
biosafety_mat_cat:
name: biosafety_mat_cat
description: JGI material category, labeled "Biosafety Material Category" on the
form and used for internal routing rather than as a biosafety level. The permissible
values are set by the slot's range; the isolate interfaces narrow that range
to organism kinds.
title: JGI Biosafety Material Category
notes:
- The slot name is kept to match the JGI form even though the values are categories,
not biosafety levels.
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Biosafety Material Category:
literal_form: Biosafety Material Category
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 17
alias: biosafety_mat_cat
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiMgInterface
- JgiMgLrInterface
- JgiMtInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: JgiIsolateMaterialKindEnum
required: true
isolate_meth:
name: isolate_meth
description: Method, protocol, or kit used to extract DNA or RNA from the sample.
title: Sample Isolation Method
examples:
- value: Wizard Genomic DNA Purification Kit
- value: CTAB chloroform extraction
- value: TRIzol RNA extraction
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Sample Isolation Method:
literal_form: Sample Isolation Method
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
- JGI labels this field Sample Isolation Method, but its definition is the
DNA or RNA extraction method.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 16
alias: isolate_meth
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
reference_genome:
name: reference_genome
description: Genome record to use as a reference for read alignment or assembly
scaffolding. Preferred formats are IMG taxon OID, Mycocosm/Phycocosm/ Phytozome
ID, or NCBI assembly accession (GCF_/GCA_). Free-text.
title: Reference Genome
notes:
- Local slot pending promotion to nmdc-schema; see https://github.com/microbiomedata/submission-schema/issues/447
comments:
- Provide a reference if one exists; helps JGI with assembly and QC even for de
novo projects.
examples:
- value: '2648501807'
description: IMG taxon OID
- value: GCF_000195995.2
description: NCBI RefSeq assembly accession
- value: Mycocosm:Aspni7
description: Mycocosm ID
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Reference Genome:
literal_form: Reference Genome
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 40
alias: reference_genome
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
sample_isolated_from:
name: sample_isolated_from
description: Free-text description of what the organism was isolated from.
title: Sample Isolated From
examples:
- value: soil
- value: leaf
- value: seawater
- value: single colony isolate
- value: DSM 3658
- value: Sorghum bicolor - root
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Sample Isolated From:
literal_form: Sample Isolated From
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 40
alias: sample_isolated_from
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
required: true
collection_site_or_growth_conditions:
name: collection_site_or_growth_conditions
description: Free-text description of the collection site or growth conditions
for the isolate.
title: Collection Site or Growth Conditions
comments:
- Required for RNA and methylation samples.
examples:
- value: Field
- value: Greenhouse
- value: Deep well
- value: Liquid culture from a frozen stock
- value: JGI grown culture
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Collection Site or Growth Conditions:
literal_form: Collection Site or Growth Conditions
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 41
alias: collection_site_or_growth_conditions
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
host_taxid:
name: host_taxid
annotations:
Expected_value:
tag: Expected_value
value: NCBI taxon identifier
description: NCBI taxon id of the host, e.g. 9606
title: host taxid
comments:
- Homo sapiens [NCBITaxon:9606] would be a reasonable has_raw_value
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Host NCBI Taxonomy ID:
literal_form: Host NCBI Taxonomy ID
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 48
keywords:
- host
- host.
- taxon
slot_uri: MIXS:0000250
alias: host_taxid
owner: JgiIsolateTranscriptomeInterface
domain_of:
- HostAssociatedInterface
- PlantAssociatedInterface
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
host_genus:
name: host_genus
description: Genus of the host organism that the sample was collected from.
comments:
- Free-text submitter-provided host genus. For an ontology-grounded host identification,
use `host_taxid` (MIXS:0000250) on the same class.
examples:
- value: Zea
description: GOLD organism_v2 host_name "Zea mays" (n=432 records, queried 2026-04-30)
- value: Escherichia
description: GOLD organism_v2 host_name "Escherichia coli K-12" (Go0084483 Escherichia
phage JSE, queried 2026-04-30)
in_subset:
- jgi_isolate
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Host Genus:
literal_form: Host Genus
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 45
alias: host_genus
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
host_species:
name: host_species
description: Species of the host organism that the sample was collected from.
comments:
- Free-text submitter-provided host species. For an ontology-grounded host identification,
use `host_taxid` (MIXS:0000250) on the same class.
examples:
- value: mays
description: GOLD organism_v2 host_name "Zea mays" (n=432 records, queried 2026-04-30)
- value: coli
description: GOLD organism_v2 host_name "Escherichia coli K-12" (Go0084483 Escherichia
phage JSE, queried 2026-04-30)
in_subset:
- jgi_isolate
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Host Species:
literal_form: Host Species
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 46
alias: host_species
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string
host_strain:
name: host_strain
description: Strain of the host organism that the sample was collected from.
comments:
- Free-text submitter-provided host strain. For an ontology-grounded host identification,
use `host_taxid` (MIXS:0000250) on the same class.
examples:
- value: K-12
description: GOLD organism_v2 host_name "Escherichia coli K-12" (Go0084483 Escherichia
phage JSE, queried 2026-04-30)
in_subset:
- jgi_isolate
from_schema: https://example.com/nmdc_submission_schema
structured_aliases:
Host Strain:
literal_form: Host Strain
predicate: EXACT_SYNONYM
notes:
- Exact JGI form template is access-restricted; source is the public submission
overview.
source: https://jgi.doe.gov/user-programs/pmo-overview/project-materials-submission-overview/
rank: 47
alias: host_strain
owner: JgiIsolateTranscriptomeInterface
domain_of:
- JgiIsolateCommonMixin
slot_group: jgi_isolate_section
range: string